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Vesicular Stomatitis Virus（VSV）

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Vesicular Stomatitis Virus（VSV）

Vesicular Stomatitis Virus（VSV）

Vesicular stomatitis virus (VSV) is a non-segmented, negative-strand RNA virus in the genus of Vesiculovirus of Rhabdoviridae family. The genome of VSV is approximately 11kb-12kb nucleotides which encodes nucleoprotein (N), phosphoprotein (P), matrixprotein (M), glycoprotein (G) and large polymerase protein (L). The modified VSV has been widely used as an anterograde tracer.rnGlycoprotein (G) is an essential component for VSV to anterograde transsynaptic. When removed G (VSV-∆G), it will lose the ability to cross synapses, but still can express the foreign gene in high abundance. Therefore,VSV-

Fig1 Schematic diagram of VSV trans-synapses transmission (Beier KT et al. Frontiers in Neural Circuits. 2013.)

BrainVTA has established the recombinant VSV platform based on our experienced staffs and our advanced molecular biology techniques, could offer VSV services to promote VSV-based neuronal tracing studies.

Applications

● Multisynaptic tracing from peripheral to brain center, or the lower to the higher brain region.
● Tracking the neural networks in disease or injury model.
● Changes in neural networks during neural development.

Fig.2 VSV-GFP was injected into the dorsal hippocampus of mice, and perfusion was obtained after 72 hours. There were many signals in the lumbar segment of the spinal cord, indicating that the hippocampus anterograde transsynaptic tracing into the spinal cord.（Figure provided by Dr. Fuqiang Xu's lab of Center for Brain Science at WIPM）

Popular VSV particles

Cat#	Name	Description	Price
V01001	VSV-EGFP	Anterograde, multisynaptic	Inquiry
V01002	VSV-mCherry	Anterograde, multisynaptic	Inquiry
V01003	VSV-BFP	Anterograde, multisynaptic	Inquiry
V02001	VSV-EnVA-ΔG-eGFP	Combined with AAV-TVA and AAV-VSVG, anterograde monosynaptic	Inquiry
V02002	VSV-EnVA-ΔG-mCherry	Combined with AAV-TVA and AAV-VSVG, anterograde monosynaptic	Inquiry
V03001	VSV-ΔG-EGFP	Combined with AAV-VSVG, anterograde monosynaptic	Inquiry
V03002	VSV-ΔG-mCherry	Combined with AAV-VSVG, anterograde monosynaptic	Inquiry
V03003	VSV-ΔG-taueGFP	Combined with AAV-VSVG, anterograde monosynaptic	Inquiry
V03004	VSV-ΔG-taueGFP-ferritin	Combined with AAV-VSVG, anterograde monosynaptic	Inquiry

References

Hastie E, Grdzelishvili VZ. Vesicular stomatitis virus as a flexible platform for oncolytic virotherapy against cancer. J Gen Virol. 2012 Dec;93(Pt 12):2529-45.
Bishnoi, S., Tiwari, R., Gupta, S., Byrareddy, S.N., Nayak, D. Oncotargeting by Vesicular Stomatitis Virus (VSV): Advances in cancer therapy. Viruses. 2018; 10(2): 90.
Beier KT, Saunders A, Oldenburg IA, Miyamichi K, Akhtar N, Luo L, Whelan SP, Sabatini B, Cepko CL. Anterograde or retrograde transsynaptic labeling of CNS neurons with vesicular stomatitis virus vectors. Proc Natl Acad Sci U S A. 2011 Sep 13;108(37):15414-9.

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Vesicular Stomatitis Virus（VSV）