Custom AAV Vector Design
We also provide Cloning Services to allow you to fully customize your order at a reasonable cost and with a quick turnaround time, include over express any genes (wildtype, mutant or synthetic) or to silence/knock-down/knock-out/knock-in any genes.Please contact us with your project details and our team will get in touch as soon as possible with an estimated price and projected turnaround time. You can also visit our Molecular Cloning page for more information. If you already have the ORF in plasmids, you just need to send the min- plasmid to us, then we will clone in AAV vector and package it.
Choose a suitable Serotype
Listed below are the most commonly used serotypes. Novel peptide modified serotypes can also be produced on a case-by-case basis. Don'see the needed ones, please contact us, and we will check our serotype library or custom one for you!
| Tissue | Optimal Serotype |
|---|---|
| Nervous System |
Cross BBB: AAV-PHP.eB, AAV-B10
Brain: AAV1, AAV2, AAV5, AAV8, AAV9
PNS: AAV-PHP.S, AAV9, AAV-MaCPNS1/2
Retrograde labeling: AAV2/retro, AAV11
Anterograde Trans-synaptic: AAV1
|
| Heart | AAV9 |
| Kidney | AAV2, AAVDJ, AAV8 |
| Liver | AAV8 |
| Lung | AAV6.2, AAV6, AAV6.2FF |
| Pancreas | AAV8, AAV-PAN |
| Eye |
Eye: AAV2, AAV5, AAV8, AAV9
Retina: AAV5, AAV8, AAV-Anc8OL65, AAV9, AAV-2.NN
Retinal Müller cells: AAV-ShH10, AAV-ShH10 (Y445F)
|
| Muscle |
Muscle: AAV1, AAV6, AAV7, AAV8, AAV9, MyoAAV
Smooth muscle: AAV1, AAV2, AAV5, AAV9
|
| Arthrosis | AAV1，AAV2，AAV5，AAV8 |
| Skin | AAVDJ、AAVEpo、AAV-VEGF16, AAV2, AAV5 |
| Reproductive organ | AAV2, AAV9, AAV8 |
| Vascular endothelial cell | AAV2, AAV1, AAVBI30, AAVBR1 |
| Fat | AAV8, AAV9, AAVRec2 |
| Cochlea | AAVPHP.B, Anc80L65, AAV2.7m8, AAVie, AAV5 |
| Enteric canal | AAV8, AAV2, AAV5, AAVMacPNS, AAVphps |
| Immune cell | AAV6, AAV ark313, LV-huCAR19 |
Rainbow Colors AAV
Rainbow colors AAV is a set product that combines common serotypes suitable for different organizations. If you use AAV for the first time, you can use the Rainbow colors AAV for pre-experiment. By which you can compare the infection effects of different serotypes on the target tissue, and explore the best injection method, injection site, virus dosage, etc., thereby getting more ideal experimental results.
| Rainbow Colors AAV | Serotype | Volume |
|---|---|---|
| Eyes/other organs | 1/2/5/6/8/9/DJ | 10 μL/serotype |
| Nervous system | 1/9/Retro/PHP. eB | 1/9/Retro/PHP. eB |
| Liver/Kidney | 2/8/9/DJ | 100 μL/serotype |
| Heart/Vascular/Muscle | 1/6/8/9 | 100 μL/serotype |
AAV packaging services
BrainVTA established mature AAV package platform, can provide customized, diverse and flexible package services for scientific research clients and corporate clients from 10^11vg to 10^17vg scale. We are happy to customize your order if a different volume, titer, or purification is needed.
| Scale | Timeline | Price |
|---|---|---|
| 200ul, >2×10^12 vg/ml | 3-4 weeks | $1350.00 |
| 500ul, >2×10^12 vg/ml | 3-4 weeks | $1700.00 |
| 1ml, >2×10^12 vg/ml | 3-4 weeks | $2380.00 |
| Other scale | required | required |
| Preclinical AAV packaging | required | required |
| Clinical-grade | required | required |
Quality Control
We use a validated assay for measuring physical titer (viral genome copy number) by real-time quantitative PCR using primers targeting the inserted DNA sequences such as WPRE, CMV and GFP instead of ITRs to avoid false high titers and quantified by comparison to a standard curve of a plasmid sample of known concentration. And silver staining for the purity.
293 production systerm
The triple transfection method involves co-transfecting the packaging cell line (usually HEK293T) with the recombinant AAV plasmid containing the gene of interest (GOI), a plasmid containing the essential rep and cap genes.
Patented One-bac 4.0 systerm
Our patented One-bac/sf9 4.0 AAV system makes it really possible to provide clients with highly-efficient, professional, and quality-assured AAV package services, that can facilitate large-scale rAAV production and rAAV-based gene therapy. If you have more stringent requirements for quality control, check out CRO services. For more informations, please contact us.
AAV packaging Services FAQs
-
1.What's the cloning capacity for rAAV?
AAV has a packaging capacity of 4.7Kb. If the GOI＞4.7kb, packaging risk will be existed.
-
2.Is AAV stable? What is the recommended storage temperature?
For long-term storage, AAV should be stored at -80℃. However if the virus is repeated freeze-and-thaw, it will cause significant decrease of titer. For short-term storage, AAV is stable at 4°C for up to almost 1-2 weeks.
-
3.How much plasmid do I need to provide and how to ship it, If I want to package an existing plasmid into a virus?
You just need to drop mini-extracted plasmids on filter paper or PCR tube winded with sealing film and send it to us. Plasmid concentration≥100ng/ul, the total amount is 5-10ul. The filter paper should be labeled with pencil.
-
4. What is the difference for gene expression with AAV or lentiviral vectors in CNS?
Lentiviral particles don't spread well after injection into brain because the particles are relatively large (90nm~100nm). LV also have a more toxic than AAV. In contrast, AAV particles spread more readily due to smaller size (20nm~100nm), Low immunogenicity and can achieve specific expression. Lastly, pretreatment the animal with mannitol about 15 min ahead of viral injection can aid the spread of viral particles in the brain as reported.
-
5.How long will AAV signal be observed after infecting in mice?
The signal can be detected at 2-3 weeks, peaked at 6 weeks in mice, and about 8 weeks in rats.
-
6.How long does the foreign gene express with AAV in mice?
Half a year
References
- Chen ZP, Wang S, Zhao X, Fang W, Wang Z, Ye H, Wang MJ, Ke L, Huang T, Lv P, Jiang X, Zhang Q, Li L, Xie ST, Zhu JN, Hang C, Chen D, Liu X, Yan C. Lipid-accumulated reactive astrocytes promote disease progression in epilepsy. https://www.nature.com/articles/s41593-023-01288-6
- de León Reyes NS, Sierra Díaz P, Nogueira R, Ruiz-Pino A, Nomura Y, de Solis CA, Schulkin J, Asok A, Leroy F. Corticotropin-releasing hormone signaling from prefrontal cortex to lateral septum suppresses interaction with familiar mice. Cell. 2023 Sep 14;186(19):4152-4.
