services

My Account

Hello,

My Items
- My Orders
- My Quotations
Manage Accounts
- Logout

Enquire Now

Webinar for You

AAV-Mediated Gene Transfer to Heart and Liver

Efficient transduction of vascular endothelial cell with serotype BR1 and Vec

Molecular Cloning Services

Home
Vector Services
Molecular Cloning Services

Molecular Cloning Services

BrainVTA offers comprehensive cloning services to create custom DNA constructs with 100% accuracy, providing fast delivery, various vector and marker options, and cost-effective solutions to save researchers time, effort, and frustration. Simply provide your project information, and we will deliver ready-to-use products for your research.

Technical Workflow

Step1. How to choose a suitable vector backbone?

Each viral delivery system has different characteristics, so it is important for you to choose the right vector in your research. Below is a quick comparison for some commonly used viral delivery vectors.

Viral Vector	AAV	LV	Ad
Genome	ssDNA	ssRNA (+)	dsDNA
Coat	Naked	Enveloped	Naked
Type	Non-integrating	Integrating	Non-integrating
Infection	Dividing and non-dividing cells	Dividing and non-dividing cells	Dividing and non-dividing cells
Packaging Capacity	4.9kb	6kb	7.5kb
Expression	Potentially long-lasting	Long-lasting	Transient
Immunogenicity	Very Low	Low	High
Transduction Efficiency	Moderate	Moderate	High
Biosafety	BSL-1	BSL-2	BSL-2

Step2. How to choose the promoter

BrainVTA offers various tissue-specific promoters. Listed below are the most commonly chosen promoters. Don' see the promoter of interest, please contact us. There are many other options in our library, meanwhile, custom promoter and serotype service are avaliable.

Organ	Serotype	Promoter
Nervous System	Cross BBB: AAV-PHP.eB, AAV-B10 Brain: AAV1, AAV2, AAV5, AAV8, AAV9 PNS: AAV-PHP.S, AAV9, AAV-MaCPNS1/2 Retrograde labeling: AAV2/retro, AAV11 Anterograde Trans-synaptic: AAV1	hSyn, CaMKIlα, mecp2, c-fos, TH, GFAP, GfaABC1D, CX3CR1, Cd68, L7/pcp2, MPZ, MBP，NG2, ChAT, mDIX, PV, NPY, SST(som), VGAT1/2, VGLUT2, DBH
Eye	Eye: AAV2, AAV5, AAV8, AAV9 Retina: AAV5, AAV8, AAV-Anc8OL65, AAV9, AAV-2.NN Retinal Müller cells: AAV-ShH10, AAV-ShH10 (Y445F)	CAG, CAR, Grm6
Heart	AAV9	cTnT, CS-CRM4-αMHC, CMV
Muscles	Muscle: AAV1, AAV6, AAV7, AAV8, AAV9, MyoAAV Smooth muscle: AAV1, AAV2, AAV5, AAV9 Skeletal muscle: AAV1, AAV-Anc80L65, AAV9	tMCK, MHCK7, CAG
Liver	AAV2, AAV5, AAV7, AAV8, AAV-DJ, AAV-Rh10, AAV-Anc80L65	TBG, TTR, Efl1α
Lung	AAV6, AAV-6.2, AAV-6.2FF, AAV9, AAV-DJ, AAV-Rh10	CMV, CAG
Kidney	AAV2, AAV8, AAV-DJ	CMV, CAG

Step 3. Add the GOI, Tag, reporter, linker

BrainVTA have an extensive collection of commonly used element (tags, reporters, linkers,ect) and gene in neurosciences as well as human and murine genes, so it is very easily to recombine them into viral vectors. If your GOI is out of our gene pool, BrainVTA can offer gene synthesis, mutagenesis, shRNA and CRISPR-Cas9 services to customize your order. Or if you have some materials in hand, you can send it to us, we do subclone into a custom viral vector.

Genetic Element	Available Options
Tags	6xHis, 3XFlag, HA, Myc
Localization	H2B, NLS, soma, NES, LCK, mito, axon
Linker	2A, IRES
Reporters	EGFP, mCherry, RFP, tagBFP, CFP, mCelulean, mCitrine, dsRed, Luciferase, etc

Step 4 Package into Virus Particle

If you would like your custom vector packaged into viral particles, please specify when you request a quote. Please visit our AAV, adenovirus, and lentivirus viral vector packaging services page for more information!

Ready to Order or Need a Quote?

We've made it easier than ever to place an order or request a quote for research animal models.

Place An Order / View Pricing

FAQs

Which bacterial strain does BrainVTA use for cloning its vectors? What bacterial strains can I use for propagating my plasmid obtained from BrainVTA?

We commonly used bacterial hosts strains such as DH5α and Stbl3.
Are there any limitations to which ORFs can be shuttled?

If you want to generate viral particles, you must not exceed the packaging capacity of the virus. Please note that the packaging capacity refers to the total insert size between and including the ITRs or LTRs.AAV cloning capacity: 4.7 kb. Adenovirus cloning capacity: 7.5 kb, Lentivirus cloning capacity: 6 kb

References

Chen ZP, Wang S, Zhao X, Fang W, Wang Z, Ye H, Wang MJ, Ke L, Huang T, Lv P, Jiang X, Zhang Q, Li L, Xie ST, Zhu JN, Hang C, Chen D, Liu X, Yan C. Lipid-accumulated reactive astrocytes promote disease progression in epilepsy. https://www.nature.com/articles/s41593-023-01288-6
de León Reyes NS, Sierra Díaz P, Nogueira R, Ruiz-Pino A, Nomura Y, de Solis CA, Schulkin J, Asok A, Leroy F. Corticotropin-releasing hormone signaling from prefrontal cortex to lateral septum suppresses interaction with familiar mice. Cell. 2023 Sep 14;186(19):4152-4171.e31. doi: 10.1016/j.cell.2023.08.010. Epub 2023 Sep 4. PMID: 37669667; PMCID: PMC7615103.
Fan BF, Hao B, Dai YD, Xue L, Shi YW, Liu L, Xuan SM, Yang N, Wang XG, Zhao H. Deficiency of Tet3 in nucleus accumbens enhances fear generalization and anxiety-like behaviors in mice. https://onlinelibrary.wiley.com/doi/10.1111/bpa.13080

Related Services

Protocol for Lentiviral Infection of Target Cells

Lentivirus (LVs) are single stranded RNA (sRNA) viruses, which are a subgroup of the retrovirus family. They can integrate into the host genome in both dividing and non-dividing cells, with a wide ran

LncRNA Service

Custom-Made AAVs

BrainVTA offers tailor-made AAVs for the customers and presents a wide variety of options to choose from. All customized services undergo stringent control to guarantee the quality of the viral partic

Lentivirus packaging services new

BrainVTA provide lentivirus services ranging from small-scale lentivirus packaging to large scale lentivirus packaging for in vitro and in vivo studies. We have developed a series of proprietary techn

Test service sample1

testing sample

Protocol for Lentiviral Infection of Target Cells

Protocol for generating monoclonal cell lines

A monoclonal cell line is essentially generated from an original polyclonal population, but it is required to separate from the polyclonal pool in order to create a pure, monoclonal cell line that is

Test service sample

testing purpose

Protocol for intrathecal injection

Intrathecal injection is a technique used to deliver drugs, viral vectors, or tracers directly into the cerebrospinal fluid (CSF) via the spinal canal.

Adenovirus (Ad) packaging services

Adenoviral vector has high transduction efficiency and high levels of short-term gene expression, and is widely used in cell and gene therapy applications. BrainVTA offers custom Adenovirus Service wh

Protocol for temporal vein injection

Temporal vein injection is a technique used to deliver substances systemically via the superficial temporal vein, commonly in neonatal rodents.

Protocol for Generation of Stable Cell Lines Using Lentivirus

In contrast to transient expression, stable expression allows long term, as well as defined and reproducible, expression of the gene of interest

sample protocol

short desc

CircRNA Service

Protocol for renal pelvis injection

Renal pelvis injection is a technique for delivering substances directly into the kidney’s collecting system.

Molecular Cloning Services

Protocol for culturing primary neurons

Isolation, dissociation, and culture of primary neurons from brain tissue, followed by incubation in Neurobasal medium for maturation and dendrite formation.

COVID-19 Coronavirus Research Tools

Plasmid ORFs

AAV Hepatitis B virus Diseases Tool

Vesicular Stomatitis Virus（VSV）

Protocol for intradermal injection

An intradermal injection is administered into the dermis, the layer just beneath the epidermis, using a small-gauge needle at a shallow angle (10–15°).