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LncRNA Service

LncRNA Service

As a global biotechnology company specializing in RNA biology, BrainVTA isrndedicated to offer high-quality lncRNA services for customers. Based onrnsophisticated equipment, advanced technologies and highly experiencedrnstaffs, we provide one stop solution for lncRNA research, including lncRNArnover-expression, knockdown and lncRNA knockout. Our most experiencedrnexperts will work with you to generate a custom product that best fits yourrnneeds

LncRNA over-expression Service

BrainVTA offer pAAV-LncRNA, pLV-LncRNA and AdV-LncRNA over-expression clone and virus package sevice. Different fluorescent reporter genes,
resistance genes, promoters, etc can be used flexibly. Our lncRNA experts with excellent technical skills will focus on provide you with an integrated lncRNA
research solution.

LncRNA Silence Service

Multiple siRNA sequences will be designed according to the adaptor sequence of LncRNA, specific and high efficiency shRNA will be used to clone in the vector.

LncRNA Services FAQs

  • 1.What precautions should be taken when use viral vectors to do LncRNA over-expression?

    Capacity of the vectors should be attention.

References

  • Ling-Ling Chen.( 2015) .Linking Long Noncoding RNA Localization and Function. Trends in Biochemical Sciences.

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Protocol for temporal vein injection

Temporal vein injection is a technique used to deliver substances systemically via the superficial temporal vein, commonly in neonatal rodents.

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In contrast to transient expression, stable expression allows long term, as well as defined and reproducible, expression of the gene of interest

Protocol for culturing primary neurons

Isolation, dissociation, and culture of primary neurons from brain tissue, followed by incubation in Neurobasal medium for maturation and dendrite formation.

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BrainVTA offers comprehensive cloning services to create custom DNA constructs with 100% accuracy, providing fast delivery, various vector and marker options, and cost-effective solutions to save rese

Protocol for renal pelvis injection

Renal pelvis injection is a technique for delivering substances directly into the kidney’s collecting system.

Subretinal injection is a precise method used to deliver substances such as viral vectors, stem cells, or drugs into the

Subretinal injection is a precise method used to deliver substances such as viral vectors, stem cells, or drugs into the subretinal space

Protocol for non-invasive intratracheal instillation

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Protocol for intradermal injection

An intradermal injection is administered into the dermis, the layer just beneath the epidermis, using a small-gauge needle at a shallow angle (10–15°).

Standard PCR Protocol

Denature DNA at 95°C, anneal primers at 50-65°C, extend at 72°C using Taq polymerase, repeat for 25-40 cycles. Amplifies specific DNA sequences.

Protocol for hepatic portal vein injection

Hepatic portal vein (HPV) injection is a method used to deliver substances directly into the liver via the portal circulation.