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AAV Hepatitis B virus Disease Tool
AAV Hepatitis B virus Disease Tool

AAV Hepatitis B virus Disease Tool

Hepatitis B virus (HBV) belongs to the Hepadanaviridae family. Which has highly infectious and stable through covalently closed circular DNA (cccDNA) in the cell nucleus of the host. The infection of HBV will result in acute or chronic necroinflammatory liver diseases. Moreover, chronic HBV infection is the major cause of hepatic cirrhosis and hepatocellular carcinoma (HCC), leading to high death rates. Although an effective vaccine was introduced years ago, the percentage of existing HBV carriers remains high and vaccination is not a treatment for established infections. As a result, effectiv

BrainVTA's AAV-HBV platform has a team of experienced scientists with deep knowledge of AAV-HBV production. We provide AAV particles with HBV genome DNA to establish the mouse models of HBV infection and replication. The model has three advantages: long termtransduction, fast HBV modeling, and stable expression of hepatitis B antigen, which accelerates the process of hepatitis B research and new drug development.

Applications

● Establish of hepatitis B virus infection model
● Basic research on hepatitis B virus infection
● Development of antiviral drugs

 

Available AAV-1.3XHBV Particles

For other genotypes or variants, customized service is available!

 
Cat# Name Description Price
PT-0717 rAAV8-HBV1.3-mer WT replicon Genotype D, Serotype ayw Inquiry
PT-7351 rAAV8-1.3HBV(B-adw) Genotype B, Serotype adw Inquiry
PT-7350 rAAV8-1.3XHBV(C-adr) Genotype C, Serotype adr Inquiry
PT-0717 rAAV8-HBV1.3-gtF Genotype F Inquiry
PT-0717 PT-0717-HBsAg2mut Genotype D, Serotype ayw, HBe Ag Negative Inquiry
 

Effect Verification

rAAV8-1.3xHBV was injected in C57 mice by via tail vein with the total virus is 2.3x1010vg, 5x1010vg and 10x1010vg, and the volume is 200uL, respectively.The contents of HBsAg, HBeAg and DNA markers of hepatitis B virus in serum were detected by orbital blood collection and observed continuously for 7 weeks.

Fig.2: Effect test of mouse model of hepatitis B viruss established by AAV-1.3xHBV. ( A-C, Different doses of virus were injected by the tail vein, and 5 balance tests for each dose. The content of hepatitis B virus marker in serum was detected. D-F, Detection results of hepatitis B virus markers in the highest dose group with the five mice.)

References

  • Global Hepatitis Report, 2017. WHO
  • Chronic Hepatitis B Virus Infection: Developing Drugs for Treatment Guidance for Industry. 2018 FDA
  • Christoph Seeger, and William S. Mason. Hepatitis B Virus Biology. Microbiology and Molecular Biology Reviews (2000). 51-68.
  • Haifeng Wang, Seahee Kim, and Wang-Shick Ryu. DDX3 DEAD-Box RNA Helicase Inhibits Hepatitis B Virus Reverse Transcription by Incorporation into Nucleocapsids. Journal of General Virology (2009). 5815-5824.
  • Man-Young Cha, Dong-Kyun Ryu. Stimulation of hepatitis B virus genome replication by HBx is linked to both nuclear and cytoplasmic HBx expression. Journal of General Virology (2009). 90:978-986.

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