The optical fiber implantation protocol involves surgically implanting an optical fiber into the target brain region to enable precise light delivery for optogenetic stimulation in vivo.

The optical stimulation protocol involves using light to activate optogenetically modified neurons by delivering specific wavelengths through fiber optics or LEDs

Virus infection of primary neurons involves preparing viral stocks, optimizing multiplicity of infection (MOI), exposing neurons to the virus, incubating under controlled conditions.

Cultivation of mouse cerebral cortex neurons involves isolating cortical tissue, enzymatic dissociation, plating on coated culture dishes, and maintaining in Neurobasal medium for neuronal growth

Recombinant lentivirus (LV) production involves transfecting HEK293T cells with packaging plasmids, followed by viral assembly, harvesting, and purification

Production of recombinant AAV (rAAV) involves transfecting HEK293 cells with helper plasmids, followed by viral assembly, purification, and titer determination for gene therapy applications.