Isolation, dissociation, and culture of primary neurons from brain tissue, followed by incubation in Neurobasal medium for maturation and dendrite formation.

The Tissue Culture Infectious Dose 50 (TCID₅₀) assay is a virological method used to determine the concentration of a virus required to infect 50% of cultured cells

The plaque assay is a virological method used to quantify infectious virus particles in a sample. It involves infecting a monolayer of host cells with a diluted virus solution

The RNAi protocol involves designing and introducing small interfering RNA (siRNA) or short hairpin RNA (shRNA) into cells, leading to targeted gene knockdown.