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Adenovirus (Ad) packaging services new sights

Adenovirus (Ad) packaging services new sights

Adenovirus (Ad) packaging services new sights
Molecular Cloning Services
BrainVTA offers comprehensive cloning services to create custom DNA constructs with 100% accuracy, providing fast delivery, various vector and marker options, and cost-effective solutions to save researchers time, effort, and frustration. Simply provide your project information, and we will deliver ready-to-use products for your research.

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FAQs

  • Are there any limitations to which ORFs can be shuttled?
    If you want to generate viral particles, you must not exceed the packaging capacity of the virus. Please note that the packaging capacity refers to the total insert size between and including the ITRs or LTRs.AAV cloning capacity: 4.7 kb. Adenovirus cloning capacity: 7.5 kb, Lentivirus cloning capacity: 6 kb
  • Which bacterial strain does BrainVTA use for cloning its vectors? What bacterial strains can I use for propagating my plasmid obtained from BrainVTA?
    We commonly used bacterial hosts strains such as DH5α and Stbl3.
  • What is recombinant AAV (rAAV)?
    Recombinant AAV is the artificial adeno-associated virus vector which does not contain any AAV rep and cap genes ( Rep and cap genes encode viral replication and capsid proteins, respectively). The gene of interest replace rep and cap and it is flanked by the ITRs which contain all the cis-acting elements necessary for replication and packaging.
  • What's the cloning capacity for rAAV?
    AAV has a packaging capacity of 4.7Kb. If the GOI>4.7kb. We need to chose other vector system.
  • Is recombinant AAV replication deficient?
    Yes, the rep and cap genes are provided in trans when producing the AAV particles, and only the ITRs sequences and the GOI are packaged. Additionally, the rep/cap plasmid and ITR plasmid do not share any regions of homology, which prevents the production of wild-type AAV.

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Protocol for temporal vein injection

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Isolation, dissociation, and culture of primary neurons from brain tissue, followed by incubation in Neurobasal medium for maturation and dendrite formation.

Molecular Cloning Services

BrainVTA offers comprehensive cloning services to create custom DNA constructs with 100% accuracy, providing fast delivery, various vector and marker options, and cost-effective solutions to save rese

Protocol for renal pelvis injection

Renal pelvis injection is a technique for delivering substances directly into the kidney’s collecting system.

Subretinal injection is a precise method used to deliver substances such as viral vectors, stem cells, or drugs into the

Subretinal injection is a precise method used to deliver substances such as viral vectors, stem cells, or drugs into the subretinal space

Protocol for non-invasive intratracheal instillation

Non-invasive intratracheal instillation is a technique used to deliver substances directly into the lungs without surgical intervention.

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An intradermal injection is administered into the dermis, the layer just beneath the epidermis, using a small-gauge needle at a shallow angle (10–15°).

Standard PCR Protocol

Denature DNA at 95°C, anneal primers at 50-65°C, extend at 72°C using Taq polymerase, repeat for 25-40 cycles. Amplifies specific DNA sequences.

Protocol for hepatic portal vein injection

Hepatic portal vein (HPV) injection is a method used to deliver substances directly into the liver via the portal circulation.